New records of copepods are listed from artificial containers and shallow waterbodies in the Florida Keys. Mesocyclops ogunnus is reported from the United States for the first time. Metacyclops cf. gracilis is reported from the Florida Keys for the first time. New collection records are presented for 12 other species. Mesocyclops ogunnus is an Old World species, and this is the third record in the Neotropics; it may have the potential to compete with the North and Central American native Mesocyclops edax. The non-native copepod species found in central and southern Florida are generally associated with plants and soils. A review of literature on introduced copepods found in South Florida is presented.